Immobilization of catalase from Aspergillus niger in calcium alginate gel
Justyna MIŁEK, Sylwia KWIATKOWSKA-MARKS, Marek WÓJCIK – Faculty of Chemical Technology and Engineering, University of Technology and Life Sciences, Bydgoszcz

Please cited as: CHEMIK 2011, 65, 4, 305-308

The entrapment of catalase from Aspergillus niger in a gel containing 2% and 4% by weight of calcium alginate was investigated. For the 2% gel, the biocatalyst was observed to gradually lose its activity as catalase was released from the gel pores. A stable biocatalyst with a maximum activity at 40?C and pH 7 was obtained using the gel containing 4% calcium alginate. The immobilized biocatalyst was found to be useful in repeated applications for decomposing H2O2 in a batch reactor.

Keywords: Catalase, immobilization, calcium alginate, entrapment