The use of components of the extract from the leaves of walnut Juglans regia L against the fungus Ascosphaera apis
GARBACZEWSKA S., CIENIECKA-ROSŁONKIEWICZ A., MICHALCZYK A., KIEŁCZEWSKA A.

Please cite as: CHEMIK 2014, 68, 2, 117–122

Ascosphaera apis is the fungal cause of the honey bee larvae disease chalkbrood. The influence of the extract from the leaves of the walnut (Juglans regia L) and obtained by the chromatographic separation fraction of the linear A. apis mycelium growth in vitro were tested. The antifungal activity of extract was weak (45%) but one obtained fraction showed 80% inhibition of fungal growth. The active components and relative contents in most active fraction were analyzed by GC-MS, DI-MS and IR. The juglone –active components present in an amount of 0.3% were tested. The minimum inhibitory concentration (MIC) test activity of juglone against A. apis was determined and was equal 2500 mg/l. In the active fraction dominated δ-tocopherol (58.6% by GC-MS). The activity δ-tocopherol against A. apis were tested, but we didn’t observed inhibit mycelium growth. The eugenol (4.6% by GC-MS) identified in active fraction also were tested with MIC values in the order of 10 000 mg/l.

Keywords: Ascosphaera apis, juglone, leaf extract of walnut, eugenol, δ-tocopherol

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