Errors in the studies of enzymatic reactions resulting from unforeseen oxidation-reduction reactions
GĄSOWSKA-BAJGER B., WOJTASEK h.

Please cite as: CHEMIK 2014, 68, 4, 341–346

Enzymatic reactions are usually monitored using spectrophotometric measurements at one wavelength. Such measurements usually pose no risk, if a standard substrate that gives known product is used. However, if a mixture of compounds is studied, e.g. a substrate and potential inhibitors, this may lead to serious errors. In such cases unexpected reactions often occur. The potential inhibitors might be themselves enzyme substrates or might react with the product or intermediates of substrate conversion. Therefore, the expected product might not be produced or by-products are formed with spectra completely differently from the expected product or overlapping with this spectrum what might give totally false results.

Keywords: oxidoreductases, tyrosinase, peroxidase, o-quinones, hydrazine, pteridines

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